A. Sultana, M.M. Islam, L. Hassan, R. Rahman and M.M. Haque
Development and continuation of the dual tolerance for salinity and submergence is a fruitful entry point for improving the rice productivity in coastal areas. A major quantitative trait locus (QTL) was identified bestowing salt tolerance on chromosome 1 and designated Saltol. Submergence tolerance is controlled by a single major QTL (Sub1) on chromosome 9. This study aims to unite Saltol and Sub1 genes within one genetic background. Crosses have been made between one advanced line (RC-251) of International Rice Research Institute as donor (salinity-submergence tolerant) with popular salt tolerant rice variety, Binadhan-10 as recurrent parent. A total of 25 microsatellite markers for Saltol and 15 markers for Sub1 have been screened for hybridization test and foreground selection. Total 80 heterozygous genotypes have been selected by hybridization test with highly polymorphic markers RM7075 (for Saltol) and RM5688 (for Sub1). Selected F1 progenies have been backcrossed and indentified a total number of 54 BC1F1 genotypes, genotyped with tightly linked salt tolerant markers (RM7075, RM490 and RM493) and submergence tolerant markers (RM5688, RM23662 and SC3). These selected genotypes be used for further back crossing and MAS for the development of salinity and submergence tolerant rice varieties to cope with common abiotic stresses such as salinity-submergence in coastal areas.
Key words: Marker-assisted selection, QTL, SSRs, Salinity Submergence and Rice.